Journal: Molecular cell
Article Title: Correlating transcription initiation and conformational changes by a single subunit RNA polymerase with near base pair resolution
doi: 10.1016/j.molcel.2018.04.018
Figure Lengend Snippet: Key Resources Table
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BL21/pAR1219 Studier FW N/A BL21/pAR1219 cyslight V174 This paper N/A Biological Samples T7 RNA polymerase This paper N/A V174C Cyslight T7 RNA polymerase This paper N/A Chemicals, Peptides, and Recombinant Proteins SP Sephadex Sigma {"type":"entrez-protein","attrs":{"text":"SPC25120","term_id":"1443690338","term_text":"SPC25120"}} SPC25120 CM-sephadex Sigma SP DEAE-sephacel GE healthcare GE17-0500-01 γ[ 32 P]GTP Perkin Elmer NEG004Z 40% Acrylamide/Bis solution Biorad 1610148EDU Cy3-NHS/Cy5-NHS GE Healthcare, Chicago, IL PA13101/PA15101 mPEG/biotin PEG Laysan Bio, Arab, AL MPEG-SC-5000/Biotin-PEG-SC-5000 Neutravidin Pierce, Rockford, IL 31000 Trolox Sigma-Aldrich, St. Louis, MO 238813 Glucose oxidase Sigma-Aldrich, St. Louis, MO G2133 Catalase Roche, Indianapolis, IN 10106810001 NTP Roche, Indianapolis, IN 11277057001 dNTP TriLink, San Diego, CA K1007 Oligonucleotides NT1, NT2, NT5, T1, T2, T5, biotin18, phi10 tempate, phi10 nontemplate (Refer to the supplementary for the sequence information) Integrated DNA Technologies, Coralville, IA Custom order Software and Algorithms Data acquisition and analysis software https://cplc.illinois.edu/software/ Home-built Open in a separate window Key Resources Table
Techniques: Recombinant, Sequencing, Software